KEPADATAN SEL DAN KADAR BETA-KAROTEN MIKROALGA HALOFILIK Dunaliella salina HASIL KULTIVASI PADA SALINITAS BERBEDA DAN PENGERINGAN BEKU

Authors

  • Aldy Nur Faqih Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Jalan Raya Telang PO Box 2 Kamal, Bangkalan 69162, Jawa Timur, Indonesia
  • Eka Nurrahema Ning Asih Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Jalan Raya Telang PO Box 2 Kamal, Bangkalan 69162, Jawa Timur, Indonesia
  • Makhfud Efendy Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Jalan Raya Telang PO Box 2 Kamal, Bangkalan 69162, Jawa Timur, Indonesia
  • Mohamad Zaki Mahasin Kementerian Kelautan dan Perikanan, Jalan Medan Merdeka Timur No.16, Jakarta Pusat 10110, DKI Jakarta, Indonesia
  • Siska Aprilliyanti Balai Besar Perikanan Budidaya Air Payau Jepara, Jalan Cik Lanang, Jepara 59418, Jawa Tengah, Indonesia

DOI:

https://doi.org/10.15578/jra.21.1.2026.53-67

Keywords:

beta-karoten, Dunaliella salina, mikroalga halofilik, salinitas berbeda, beta-carotene, halophilic microalgae, varying salinity

Abstract

Dunaliella salina merupakan mikroalga halofilik uniseluler hijau yang tumbuh pada salinitas ekstrem. Pertumbuhan sel D. salina dan kandungan beta-karotennya sangat bergantung pada salinitas media. Penelitian ini bertujuan untuk mengevaluasi pengaruh salinitas terhadap kepadatan sel dan kandungan beta-karoten D. salina. Penelitian ini dirancang dengan model rancangan acak lengkap dengan menggunakan empat tingkat salinitas, yaitu 15, 30, 45, dan 60 ppt. Hasil kultur D. salina dikeringkan dengan menggunakan metode freeze drying pada suhu beku -50°C dan diuji menggunakan metode spektrofotometri untuk mengetahui kandungan beta-karotennya. Hasil penelitian ini menunjukkan bahwa terdapat perbedaan yang signifikan (p < 0,05) pada kepadatan sel D. salina dengan perlakuan salinitas media kultur yang berbeda. Kepadatan sel D. salina tertinggi didapati pada salinitas 15 ppt (142,83 × 104 sel mL-1), sedangkan kepadatan sel terendah terdapat pada salinitas 60 ppt (103,16 × 104 sel mL-1). Variasi salinitas secara signifikan dapat memengaruhi kandungan beta-karoten D. salina (p < 0,05). Perlakuan salinitas ekstrem (60 ppt) mampu menghasilkan rata-rata kandungan beta-karoten sebesar 1,320 ± 0,01 µg g-1, sedangkan perlakuan salinitas terendah (15 ppt) menghasilkan rata-rata kandungan beta-karoten sebesar 0,074 ± 0,01 µg g-1. Pada kesimpulannya, temuan dalam penelitian ini mengindikasikan bahwa salinitas merupakan faktor kunci yang meregulasi pertumbuhan D. salina dan akumulasi beta-karotennya, dengan salinitas rendah (15 ppt) membantu dalam pertumbuhan sel dan salinitas tinggi (60 ppt) dapat meningkatkan produksi beta-karoten. 

Dunaliella salina is a green unicellular halophilic microalga that grows in extreme salinity. The cell growth of D. salina and its beta-carotene content are highly influenced on the salinity of the culture media. This study aimed to evaluate the effects of salinity on cell density and beta-carotene content of D. salina. This experiment was conducted using a completely randomized design with four salinity treatments: 15, 30, 45, and 60 ppt. The biomass of D. salina was dried using freeze drying method at -50°C and tested by spectrophotometry method to determine the beta-carotene content. The results demonstrated that salinity significantly affected the cell density of D. salina (p < 0.05). The highest cell density of D. salina was found at a salinity of 15 ppt (142.83 × 104 cells mL-1), while the lowest cell density was found at a salinity of 60 ppt (103.16 × 104 cells mL-1). Variations in salinity could significantly influence the beta-carotene content of D. salina (p < 0.05). The extreme salinity treatment (60 ppt) was able to produce an average beta-carotene content of 1.320 ± 0.01 µg g-1, while the lowest salinity treatment (15 ppt) produced an average beta-carotene content of 0.074 ± 0.01 µg g-1. In conclusion, these findings indicate that salinity is a key factor regulating the growth and beta-carotene accumulation of D. salina, with low salinity (15 ppt) favoring cell growth and high salinity (60 ppt) promoting beta-carotene production.

Author Biographies

  • Aldy Nur Faqih, Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Jalan Raya Telang PO Box 2 Kamal, Bangkalan 69162, Jawa Timur, Indonesia

    Departemen Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Bangkalan 69162, Jawa Timur, Indonesia

  • Eka Nurrahema Ning Asih, Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Jalan Raya Telang PO Box 2 Kamal, Bangkalan 69162, Jawa Timur, Indonesia

    Departemen Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Bangkalan 69162, Jawa Timur, Indonesia

  • Makhfud Efendy, Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Jalan Raya Telang PO Box 2 Kamal, Bangkalan 69162, Jawa Timur, Indonesia

    Departemen Ilmu Kelautan, Fakultas Pertanian, Universitas Trunodjoyo Madura, Bangkalan 69162, Jawa Timur, Indonesia

  • Mohamad Zaki Mahasin, Kementerian Kelautan dan Perikanan, Jalan Medan Merdeka Timur No.16, Jakarta Pusat 10110, DKI Jakarta, Indonesia

    Kementerian Kelautan dan Perikanan, Gedung Mina Bahari IV Lt 1, Jl. Medan Merdeka Timur No. 16, Jakarta Pusat 10041, Indonesia

     

  • Siska Aprilliyanti, Balai Besar Perikanan Budidaya Air Payau Jepara, Jalan Cik Lanang, Jepara 59418, Jawa Tengah, Indonesia
    Balai Besar Perikanan Budidaya Air Payau, Jl. Cik Lanang, Kelurahan Bulu, Kecamatan Jepara, Kabupaten Jepara, Jawa Tengah 59418, Indonesia

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2026-03-31

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Vol 21, No 1 (2026): Maret (2026)

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