Color Stability of Phycoerythrin Crude Extract (PECE) from Rhodomonas Salina Toward Physicochemical Factors
Penulis
Endar Marraskuranto
Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
Badan Riset dan Sumber Daya Manusia
Kementerian Kelautan dan Perikanan
Penulis
Tri Joko Raharjo
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Jl. Bulaksumur, Sleman, DI Yogyakarta, Indonesia
Penulis
Rina Sri Kasiamdari
Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Bulaksumur, Sleman, DI Yogyakarta, Indonesia
Penulis
Tri Rini Nuringtyas
Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Bulaksumur, Sleman, DI Yogyakarta, Indonesia
Penulis
Rhodomonas salina produces Cr-phycoerythrin545 as its designated phycoerythrin (PE) with an absorption maximum at 545 nm and a shoulder 564 nm. PE has potential to be applied as colorants, pharmaceutical agents, and fluorescent dye tags. The stability of the PE color is influenced by the physicochemical factors of the solution. This study aimed to analyze the color stability of PECE against chemical (ethanol and pH) and physical (light and temperature) factors. PECE was prepared from freeze-dried biomass of R. salina and was extracted in phosphate buffer solution (pH = 6.0) using a freeze-thaw method in -25 oC (2 hours) and 4 oC (24 hours). The resulting extract was concentrated and dried in a freeze-dryer. Analyses were conducted using UV-visible and fluorescence spectrophotometer. PECE showed color stability against light of white fluorescent lamp exposure up to 8 hours, temperature exposure up to 40 oC, ethanol solution up to concentration of 20 % (v/v), and pH range 3.9-8.42. Results from this study can be useful for extraction, purification, and future application of Cr-PE545.
